abstract：:Understanding in vivo regulation of photoautotrophic metabolism is important for identifying strategies to improve photosynthetic efficiency or re-route carbon fluxes to desirable end products. We have developed an approach to reconstruct comprehensive flux maps of photoautotrophic metabolism by computational analysis...

journal_title：Metabolic engineering

authors： Young JD,Shastri AA,Stephanopoulos G,Morgan JA

更新日期：2011-11-01 00:00:00

abstract：:Saccharomyces cerevisiae lacks the ability to ferment the pentose sugar xylose that is the second most abundant sugar in nature. Therefore two different xylose catabolic pathways have been heterologously expressed in S. cerevisiae. Whereas the xylose reductase (XR)-xylitol dehydrogenase (XDH) pathway leads to the prod...

journal_title：Metabolic engineering

authors： Parachin NS,Bergdahl B,van Niel EW,Gorwa-Grauslund MF

更新日期：2011-09-01 00:00:00

abstract：:Glucose pulse experiments at seconds time scale resolution were performed in aerobic glucose-limited Escherichia coli chemostat cultures. The dynamic responses of oxygen-uptake and growth rate at seconds time scale were determined using a new method based on the dynamic liquid-phase mass balance for oxygen and the pse...

journal_title：Metabolic engineering

authors： Taymaz-Nikerel H,van Gulik WM,Heijnen JJ

更新日期：2011-05-01 00:00:00

abstract：:Having previously introduced the mathematical framework of topological metabolic analysis (TMA) - a novel optimization-based technique for modeling metabolic networks of arbitrary size and complexity - we demonstrate how TMA facilitates unique methods of metabolic interrogation. With the aid of several hybridoma metab...

journal_title：Metabolic engineering

authors： Baughman AC,Sharfstein ST,Martin LL

更新日期：2011-03-01 00:00:00

abstract：:To synthesize glycerol, a major by-product during anaerobic production of ethanol, the yeast Saccharomyces cerevisiae would consume up to 4% of the sugar feedstock in typical industrial ethanol processes. The present study was dedicated to decreasing the glycerol production mostly in industrial ethanol producing yeast...

journal_title：Metabolic engineering

authors： Guo ZP,Zhang L,Ding ZY,Shi GY

更新日期：2011-01-01 00:00:00

abstract：:One of the challenges in strain improvement by evolutionary engineering is to subsequently determine the molecular basis of the improved properties that were enriched from the natural genetic variation during the selective conditions. This study focuses on Saccharomyces cerevisiae IMS0002 which, after metabolic and ev...

journal_title：Metabolic engineering

authors： Wisselink HW,Cipollina C,Oud B,Crimi B,Heijnen JJ,Pronk JT,van Maris AJ

更新日期：2010-11-01 00:00:00

abstract：:A large number of CHO glycosylation mutants were isolated by Ricinus communis agglutinin-I (RCA-I). Complementation tests revealed that all these mutant lines possessed a dysfunctional N-acetylglucosaminyltransferase I (GnT I) gene. Sequencing analyses on the GnT I cDNAs isolated from 16 mutant lines led to the identi...

journal_title：Metabolic engineering

authors： Goh JS,Zhang P,Chan KF,Lee MM,Lim SF,Song Z

更新日期：2010-07-01 00:00:00

abstract：:To investigate primary effects of a pyruvate kinase (PYK) defect on glucose metabolism in Corynebacterium glutamicum, a pyk-deleted mutant was derived from wild-type C. glutamicum ATCC13032 using the double-crossover chromosome replacement technique. The mutant was then evaluated under glutamic acid-producing conditio...

journal_title：Metabolic engineering

authors： Sawada K,Zen-in S,Wada M,Yokota A

更新日期：2010-07-01 00:00:00

abstract：:Cell culture engineering has to-date used transcriptomic, proteomic, and metabolic flux analyses, attempting to resolve significant questions regarding cell culture performance. Despite the foreseen positive impact, the metabolomic analytical platform has not yet been vastly deployed. Presently, there is no published ...

journal_title：Metabolic engineering

authors： Chrysanthopoulos PK,Goudar CT,Klapa MI

更新日期：2010-05-01 00:00:00

abstract：:We generated a genomic library from sheared Clostridium acetobutylicum ATCC 824 DNA, whereby inserts can be expressed in both directions from the thiolase promoter, P(thl). Serial transfer of library-bearing C. acetobutylicum cultures exposed to increasing butyrate concentrations enriched for inserts containing fragme...

journal_title：Metabolic engineering

authors： Borden JR,Jones SW,Indurthi D,Chen Y,Papoutsakis ET

更新日期：2010-05-01 00:00:00

abstract：:One of the main strategies to improve the production of relevant metabolites has been the manipulation of single or multiple key genes in the metabolic pathways. This kind of strategy requires several rounds of experiments to identify enzymes that impact either yield or productivity. The use of mathematical tools to f...

journal_title：Metabolic engineering

authors： Contador CA,Rizk ML,Asenjo JA,Liao JC

更新日期：2009-07-01 00:00:00

abstract：:An innovative "biodrug" concept based on oral administration of living recombinant microorganisms as a vehicle to deliver active compounds directly into the digestive tract has recently been developed. To validate this concept, we studied a recombinant Saccharomyces cerevisiae strain in order to investigate its viabil...

journal_title：Metabolic engineering

authors： Garrait G,Jarrige JF,Blanquet-Diot S,Alric M

更新日期：2009-05-01 00:00:00

abstract：:The complete genome sequence of the filamentous fungi Aspergillus nidulans has paved the way for fundamental research on this industrially important species. To the best of our knowledge, this is the first time a gene encoding for ATP-dependent NADH kinase (ATP:NADH 2'-phosphotransferase, EC 2.7.1.86) has been identif...

journal_title：Metabolic engineering

authors： Panagiotou G,Grotkjaer T,Hofmann G,Bapat PM,Olsson L

更新日期：2009-01-01 00:00:00

abstract：:Whereas microbial fermentation processes for producing ethanol and related alcohol biofuels are well established, biodiesel (methyl esters of fatty acids) is exclusively derived from plant oils. Slow cycle times for engineering oilseed metabolism and the excessive accumulation of glycerol as a byproduct are two major ...

journal_title：Metabolic engineering

authors： Lu X,Vora H,Khosla C

更新日期：2008-11-01 00:00:00

abstract：:Geobacter species are among the most effective microorganisms known for the bioremediation of radioactive and toxic metals in contaminated subsurface environments and for converting organic compounds to electricity in microbial fuel cells. However, faster rates of electron transfer could aid in optimizing these proces...

journal_title：Metabolic engineering

authors： Izallalen M,Mahadevan R,Burgard A,Postier B,Didonato R Jr,Sun J,Schilling CH,Lovley DR

更新日期：2008-09-01 00:00:00

abstract：:A methionine-producing strain was derived from a lysine-producing Corynebacterium glutamicum through a process of genetic manipulation in order to assess its potential to synthesize and accumulate methionine during growth. The strain carries a deregulated hom gene (hom(FBR)) to abolish feedback inhibition of homoserin...

journal_title：Metabolic engineering

authors： Park SD,Lee JY,Sim SY,Kim Y,Lee HS

更新日期：2007-07-01 00:00:00

abstract：:Two types of non-homologous beta-carotene ketolases (CrtW and CrtO) were previously described. We report improvement of a CrtO-type of beta-carotene ketolase for canthaxanthin production in a methylotrophic bacterium, Methylomonas sp. 16a, which could use the C1 substrate (methane or methanol) as sole carbon and energ...

journal_title：Metabolic engineering

authors： Tang XS,Shyr J,Tao L,Sedkova N,Cheng Q

更新日期：2007-07-01 00:00:00

abstract：:A 2D-NMR method based on zero-quantum filtered (ZQF-) TOtal Correlation SpectroscopY (TOCSY) was applied to measure 13C-enrichments in complex mixtures of 13C-labeled metabolites generated in carbon-labeling experiments. Using ZQF-TOCSY, more than 30 13C-enrichments could be potentially measured from the analysis of a...

journal_title：Metabolic engineering

authors： Massou S,Nicolas C,Letisse F,Portais JC

更新日期：2007-05-01 00:00:00

abstract：:The chemical monomer p-hydroxystyrene (pHS) is used for producing a number of important industrial polymers from petroleum-based feedstocks. In an alternative approach, the microbial production of pHS can be envisioned by linking together a number of different metabolic pathways, of which those based on using glucose ...

journal_title：Metabolic engineering

authors： Qi WW,Vannelli T,Breinig S,Ben-Bassat A,Gatenby AA,Haynie SL,Sariaslani FS

更新日期：2007-05-01 00:00:00

abstract：:Discovery of the cold-inducible RNA-binding protein (CIRP) in mouse fibroblasts suggests that growth suppression at hypothermic conditions is due to an active response by the cell rather than due to passive thermal effects. To determine the effect of down-regulated CIRP expression on cell growth and erythropoietin (EP...

journal_title：Metabolic engineering

authors： Hong JK,Kim YG,Yoon SK,Lee GM

更新日期：2007-03-01 00:00:00

abstract：:Previous metabolic engineering strategies for improving glycerol production by Saccharomyces cerevisiae were constrained to a maximum theoretical glycerol yield of 1 mol.(molglucose)(-1) due to the introduction of rigid carbon, ATP or redox stoichiometries. In the present study, we sought to circumvent these constrain...

journal_title：Metabolic engineering

authors： Geertman JM,van Maris AJ,van Dijken JP,Pronk JT

更新日期：2006-11-01 00:00:00

abstract：:In this study strains of Ralstonia eutropha H16 and Pseudomonas putida KT2440 were engineered which are suitable for biotechnological production of 2-methylcitric acid (2MC). Analysis of a previous mutant of R. eutropha able to accumulate 2MC recommended this strain as a candidate for fermentative production of 2MC. T...

journal_title：Metabolic engineering

authors： Ewering C,Heuser F,Benölken JK,Brämer CO,Steinbüchel A

更新日期：2006-11-01 00:00:00

abstract：:Selective estrogen receptor (ER) modulators are highly successful breast cancer therapies, but they are not effective in patients with ER negative and selective estrogen receptor modulator (SERM)-resistant tumors. Understanding the mechanisms of estrogen-stimulated proliferation may provide a route to design estrogen-...

journal_title：Metabolic engineering

authors： Forbes NS,Meadows AL,Clark DS,Blanch HW

更新日期：2006-11-01 00:00:00

abstract：:(13)C metabolic flux analysis (MFA) has become an important and powerful tool for the quantitative analysis of metabolic networks in the framework of metabolic engineering. Isotopically instationary (13)C MFA under metabolic stationary conditions is a promising refinement of classical stationary MFA. It accounts for t...

journal_title：Metabolic engineering

authors： Nöh K,Wahl A,Wiechert W

更新日期：2006-11-01 00:00:00

abstract：:As a proof of concept, the qualitative and quantitative engineering of carotenoid formation has been achieved in crop plants. Successful reports in tomato, potato, rice, and canola all describe the enhancement of carotenoid with nutritional value, while in model systems such as tobacco and Arabidopsis the engineering ...

journal_title：Metabolic engineering

authors： Sandmann G,Römer S,Fraser PD

更新日期：2006-07-01 00:00:00

abstract：:Presented herein is a methodology for quantitatively analyzing the complex signaling network by resorting to colored Petri nets (CPN). The mathematical as well as Petri net models for two basic reaction types were established, followed by the extension to a large signal transduction system stimulated by epidermal grow...

journal_title：Metabolic engineering

authors： Lee DY,Zimmer R,Lee SY,Park S

更新日期：2006-03-01 00:00:00

abstract：:Retroviral vectors released from mouse-derived packaging cell lines are inactivated in human sera by naturally occurring antibodies due to the recognition of Galalpha1,3Galbeta1,4GlcNAc (alphagal-epitope) decorated surface proteins. In this study, an extensive analysis of the glycosylation potential of NIH3T3-derived ...

journal_title：Metabolic engineering

authors： Hansen W,Grabenhorst E,Nimtz M,Müller K,Conradt HS,Wirth M

更新日期：2005-05-01 00:00:00

abstract：:The potential to produce succinate aerobically in Escherichia coli would offer great advantages over anaerobic fermentation in terms of faster biomass generation, carbon throughput, and product formation. Genetic manipulations were performed on two aerobic succinate production systems to increase their succinate yield...

journal_title：Metabolic engineering

authors： Lin H,Bennett GN,San KY

更新日期：2005-03-01 00:00:00

abstract：:Directed molecular evolution is a recursive process of controlled genetic diversification and functional screening. The success of this approach is dependent on both the quality of the genetic diversity and the ability to accurately screen a large population of individual genetic variants for those having improved fun...

journal_title：Metabolic engineering

authors： Kittell J,Borup B,Voladari R,Zahn K

更新日期：2005-01-01 00:00:00

abstract：:Metabolic engineering technology for industrial microorganisms is under development to create rational, more reliable, and more cost-effective approaches to strain improvement. Strain improvement is a critical component of the drug development process, yet the genetic basis for high production by industrial microorgan...

journal_title：Metabolic engineering

authors： Reeves AR,Cernota WH,Brikun IA,Wesley RK,Weber JM

更新日期：2004-10-01 00:00:00

abstract：:Genome-wide or large-scale methodologies employed in functional genomics such as DNA sequencing, transcription profiling, proteomics, and metabolite profiling have become important tools in many metabolic engineering strategies. These techniques allow the identification of genetic differences and insight into their ce...

journal_title：Metabolic engineering

authors： Bro C,Nielsen J

更新日期：2004-07-01 00:00:00

abstract：:Metabolic Engineering offers an opportunity to forge a link between metabolic physiologists, working with mammalian systems and metabolic engineers. Many parallels may be drawn between the specific modification of metabolic networks to improve cellular properties and the analysis of metabolic networks in search of cau...

journal_title：Metabolic engineering

authors： Kelleher JK

更新日期：2004-01-01 00:00:00

abstract：:Stress hormones and pro-inflammatory cytokines are putative signals triggering increased energy expenditure or "hypermetabolism" commonly observed in inflammatory states. Cytokines also cause the release of reactive oxidants by immune cells resident in tissues in vivo. Therefore, we hypothesized that oxidative stress ...

journal_title：Metabolic engineering

authors： Lee K,Berthiaume F,Stephanopoulos GN,Yarmush ML

更新日期：2003-10-01 00:00:00

abstract：:This paper presents a new mathematical framework for modeling of in vivo dynamics and for metabolic re-design: the linlog approach. This approach is an extension of metabolic control analysis (MCA), valid for large changes of enzyme and metabolite levels. Furthermore, the presented framework combines MCA with kinetic ...

journal_title：Metabolic engineering

authors： Visser D,Heijnen JJ

更新日期：2003-07-01 00:00:00

abstract：:A Windows program for metabolic engineering analysis and experimental design has been developed. A graphical user interface enables the pictorial, "on-screen" construction of a metabolic network. Once a model is composed, balance equations are automatically generated. Model construction, modification and information e...

journal_title：Metabolic engineering

authors： Zhu T,Phalakornkule C,Ghosh S,Grossmann IE,Koepsel RR,Ataai MM,Domach MM

更新日期：2003-04-01 00:00:00

abstract：:Understanding the metabolic and regulatory pathways of hepatocytes is important for biotechnological applications involving liver cells. Previous attempts to culture hepatocytes in plasma yielded poor functional results. Recently we reported that hormone (insulin and hydrocortisone) and amino acid supplementation redu...

journal_title：Metabolic engineering

authors： Chan C,Berthiaume F,Lee K,Yarmush ML

更新日期：2003-01-01 00:00:00

abstract：:Using the carbon isotope labeling technique, the response of cyanobacterial central carbon metabolism to the change in environmental conditions was investigated. Synechocystis was grown in the heterotrophic and mixotrophic cultures fed with 13C-labeled glucose. The labeling patterns of the amino acids in biomass hydro...

journal_title：Metabolic engineering

authors： Yang C,Hua Q,Shimizu K

更新日期：2002-07-01 00:00:00

abstract：:Fatty acids are the most abundant form of reduced carbon chains available from nature and have diverse uses ranging from food to industrial feedstocks. Plants represent a significant renewable source of fatty acids because many species accumulate them in the form of triacylglycerol as major storage components in seeds...

journal_title：Metabolic engineering

authors： Thelen JJ,Ohlrogge JB

更新日期：2002-01-01 00:00:00

abstract：:A mathematical model to describe carbon catabolite repression in Escherichia coli is developed and in part validated. The model is aggregated from two functional units describing glucose and lactose transport and degradation. Both units are members of the crp modulon and are under control of a global signal transducti...

journal_title：Metabolic engineering

authors： Kremling A,Bettenbrock K,Laube B,Jahreis K,Lengeler JW,Gilles ED

更新日期：2001-10-01 00:00:00

abstract：:Based on the analysis of molecular interactions of proteins with DNA binding sites, a new approach to developing mathematical models describing gene expression is introduced. Detection of hierarchical structures in metabolic networks can be used to decompose complex reaction schemes. This will be achieved by assigning...

journal_title：Metabolic engineering

authors： Kremling A,Gilles ED

更新日期：2001-04-01 00:00:00